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SODIS in Winter

SODIS in Winter

 

1) Methods and Materials
The important guideline is that another worker of similar training and ability, following your description, should get the same results. Note that this section should be written as a description of what you did, not as a set of instructions.
2) Results
In this section you should describe the important qualitative and quantitative observations of your work. Data should be tabulated and/or graphed and described. One common error is saying, “The data are plotted in Fig. 1”, and not, “As can be seen from the graph, absorbance at 260nm is relatively constant up to about 80C, after which a sharp rise is noted.” All graphs and tables should be numbered and provided with a title.
Protocol for Class SODIS experiment

Purpose: Demonstrate the effectiveness of SODIS in the winter.

Materials:
solar reflector
9 clean 500 ml plastic PET water bottles

Methods:
1. Fill nine 500 ml plastic PET water bottles 3/4 with Versoix River water from La Batie. Shake the bottle for 20s. Then completely fill the bottle.
2. Take one bottle directly to the lab for initial E.Coli count of the raw water sample.
2. Label the remaining eight bottles: 1, 1F, 2, 2F, 3, 3F, 4, 4F. The numbers correspond to the number of days of sunlight and heat exposure. F stands for foil. The F bottles will be wrapped in foil so the samples no do receive sunlight, only heat.
3. After the first day of exposure, bring the 1 and 1F bottles to the lab for E. Coli analysis. Repeat for second, third and fourth day of exposure.

Control Treatment:
Water without UV-A exposure (sunlight) and without heat (solar reflector) – raw water sample
Water without UV-A exposure (sunlight) but with heat (solar reflector)

Data Interpretation
A graph will be used to plot the results.
The number of E. Coli (in Colony-Forming Units or CFU) will be plotted on the Y-axis (ordinate) and the exposure time (sunlight and heat) will be plotted on the X-axis.
The number of E. Coli (in Colony-Forming Units or CFU) will be plotted on the Y-axis (ordinate) and the exposure time (heat) will be plotted on the X-axis.
If the heat treatment group has fewer E. Coli than raw water sample, then can conclude that heat has an effect.
If the sunlight and heat treatment group has fewer E. Coli than the heat treatment group, then can conclude that there is a synergistic effect.

Observations from our SODIS experiments

Observations are written in lab books.
Day 1: The experiment was conducted outside for 2h13min (from 12:17pm to 2:30pm. The sky was cloudy and there was light snow furries. There was a little bit of sun. From 1pm to 1:40pm, cling wrap was placed over the opening to prevent snow from entering the solar reflector. The inside surface of the cling wrap steamed up. Hence, the cling wrap was removed. (SNOW FLURRIES)
Day 2: The experiment was conducted inside behind glass (house) because it was snowing outside. The experiment took place from 12:28pm to 4:40pm or for 2h12min. It was cloudy and there was no direct sunlight. (SNOWING)
Day 3: The experiment was conducted behind glass (car). From 11am-1pm, the sky was cloudy and the experiment was not facing the direction of the sun as the direction of the sun could not be determined. From 1pm-1:30pm, the sun came out briefly and the experiment was adjusted to face the sun. From 1:30pm to 3:10pm, there was a light rain. At 3:30pm, the experiment was stopped as there was heavy rain. (Cloudy, sunny, rainy)
Day 4: The experiment was conducted from 12:30pm to 4:30pm behind glass (house) as it was lightly snowing outside. There was lots of snow on the groud. It was bright but there was no direct sunlight. The bottles had a brown/green sediment. It was not attached to the plastic bottle, but it was denser than water. (Light snow)
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